Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human prostate cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12024-1-AP targets NUSAP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NUSAP1 fusion protein Ag2654|
|Full Name||nucleolar and spindle associated protein 1|
|Calculated molecular weight||440 aa, 49 kDa|
|Observed molecular weight||47-52 kDa|
|GenBank accession number||BC001308|
|Gene ID (NCBI)||51203|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.
|Product Specific Protocols|
|WB protocol for NUSAP1 antibody 12024-1-AP||Download protocol|
|IHC protocol for NUSAP1 antibody 12024-1-AP||Download protocol|
|IF protocol for NUSAP1 antibody 12024-1-AP||Download protocol|
|IP protocol for NUSAP1 antibody 12024-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Enhanced protein degradation by branched ubiquitin chains.
microRNA 193a-5p Regulates Levels of Nucleolar- and Spindle-Associated Protein 1 to Suppress Hepatocarcinogenesis.
Regulated degradation of spindle assembly factors by the anaphase-promoting complex.
Microtubule-dependent regulation of mitotic protein degradation.
J Exp Med
Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.
Transcriptome analysis of MENX-associated rat pituitary adenomas identifies novel molecular mechanisms involved in the pathogenesis of human pituitary gonadotroph adenomas.