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NUSAP1 Polyclonal antibody

NUSAP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 12024-1-AP


ANKT, BM037, LNP, NuSAP, NUSAP1, PRO0310, PRO0310p1, Q0310, SAPL

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12024-1-AP targets NUSAP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUSAP1 fusion protein Ag2654
Full Name nucleolar and spindle associated protein 1
Calculated molecular weight 440 aa, 49 kDa
Observed molecular weight 47-52 kDa
GenBank accession numberBC001308
Gene symbol NUSAP1
Gene ID (NCBI) 51203
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.


Product Specific Protocols
WB protocol for NUSAP1 antibody 12024-1-APDownload protocol
IHC protocol for NUSAP1 antibody 12024-1-APDownload protocol
IF protocol for NUSAP1 antibody 12024-1-APDownload protocol
IP protocol for NUSAP1 antibody 12024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Enhanced protein degradation by branched ubiquitin chains.

Authors - Hermann-Josef Meyer


microRNA 193a-5p Regulates Levels of Nucleolar- and Spindle-Associated Protein 1 to Suppress Hepatocarcinogenesis.

Authors - Sanchari Roy

Mol Cell

Regulated degradation of spindle assembly factors by the anaphase-promoting complex.

Authors - Ling Song

Mol Cell

Microtubule-dependent regulation of mitotic protein degradation.

Authors - Ling Song
humanIHC, IF

J Exp Med

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

Authors - Yalong Wang
human, ratIHC

Acta Neuropathol

Transcriptome analysis of MENX-associated rat pituitary adenomas identifies novel molecular mechanisms involved in the pathogenesis of human pituitary gonadotroph adenomas.

Authors - Lee Misu M