NaPi-IIa Polyclonal antibody

NaPi-IIa Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26783-1-AP

Synonyms

Na(+)/Pi cotransporter 2A, NaPi 2a, NAPI 3, napi2a, NaPi-IIa, SLC11, SLC17A2, SLC34A1

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Validation Data Gallery

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  WB analysis of mouse kidney using 26783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26783-1-AP (NaPi-IIa antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse lung tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26783-1-AP targets NaPi-IIa in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NaPi-IIa fusion protein Ag24807
• Full Name: solute carrier family 34 (sodium phosphate), member 1
• Observed Molecular Weight: 70-75 kDa, 30-35 kDa
• GenBank Accession Number: BC053349
• Gene Symbol: SLC34A1
• Gene ID (NCBI): 6569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NaPi-IIa, also known as Npt2a (SLC34A1), is an electrogenic Na+-dependent Pi cotransporter expressed in the brush border membranes (BBM) of renal proximal tubules. NaPi-IIa could be detected with an apparent molecular weight 75 kDa, corresponding to the full length glycosylated protein, together with a lower molecular weight 37 kDa known to be a N-terminal proteolytic fragment which is also glycosylated (PMID:36074191).