Nephrin Monoclonal antibody, PBS Only

Nephrin Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D11H8

Cat no : 66970-1-PBS

Synonyms

CNF, Nephrin, NPHS1

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Validation Data Gallery

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  IHC staining of human kidney using 66970-1-Ig (same clone as 66970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66970-1-Ig (Nephrin antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66970-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66970-1-Ig (same clone as 66970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66970-1-Ig (Nephrin antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66970-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66970-1-PBS targets Nephrin in IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Nephrin fusion protein Ag19273
• Full Name: nephrosis 1, congenital, Finnish type (nephrin)
• Calculated Molecular Weight: 1241 aa, 135 kDa
• GenBank Accession Number: BC156935
• Gene Symbol: NPHS1
• Gene ID (NCBI): 4868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.