OAT1 Polyclonal antibody

OAT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 26574-1-AP

Synonyms

HOAT1, hPAHT, hROAT1, OAT1, Organic anion transporter 1, PAH transporter, PAHT, ROAT1, SLC22A6

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Validation Data Gallery

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  WB analysis of mouse brain using 26574-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26574-1-AP (OAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 26574-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26574-1-AP (OAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26574-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26574-1-AP (OAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

The immunogen of 26574-1-AP is OAT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OAT1 fusion protein Ag24234
• Full Name: solute carrier family 22 (organic anion transporter), member 6
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 60-65 and 70-80 kDa
• GenBank Accession Number: BC033682
• Gene Symbol: OAT1
• Gene ID (NCBI): 9356
• RRID: AB_2880557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OAT1, also named as SLC22A6, has two GXXXG motifs in its transmembrane which is associated with protein processing and oligomerization of other proteins. OAT1 is involved in the renal elimination of endogenous and exogenous organic anions. It has been reported that OAT1 plays a key role in clearing endogenous metabolites, toxins and drugs from blood. OAT1 has some isoforms and the calculated MW ranges from 55 kDa to 62 kDa. 26574-1-AP antibody detects the 60-65 kDa (native forms) and 70-80 kDa (mature forms) protein in SDS-PAGE. (PMID: 21340049, 23389457, 23196129)

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice; Authors - Xiao-Li Guo
mouse	WB	J Ethnopharmacol; Fuling-Zexie formula attenuates hyperuricemia-induced nephropathy and inhibits JAK2/STAT3 signaling and NLRP3 inflammasome activation in mice; Authors - Meixi Lu
mouse	WB	J Ethnopharmacol; Simiao San alleviates hyperuricemia and kidney inflammation by inhibiting NLRP3 inflammasome and JAK2/STAT3 signaling in hyperuricemia mice; Authors - Yueyi Zhang
mouse	WB	Am J Physiol Renal Physiol; SGLT2 inhibitor dapagliflozin protects the kidney in a murine model of Balkan nephropathy; Authors - Yuji Oe
human	WB	Tissue Eng Part A; Inhibition of TGF-β improves primary renal tubule cell differentiation in long-term culture; Authors - Kuniko Hunter
mouse	WB	Eur J Pharmacol; Berberrubine attenuates potassium oxonate- and hypoxanthine-induced hyperuricemia by regulating urate transporters and JAK2/STAT3 signaling pathway; Authors - Guoshu Lin