OCT2-Specific Polyclonal antibody, PBS Only

• 

  WB analysis of mouse thymus using 18996-1-AP (same clone as 18996-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 18996-1-AP (same clone as 18996-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 18996-1-AP (same clone as 18996-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 18996-1-AP (same clone as 18996-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 18996-1-AP (same clone as 18996-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 18996-1-AP (same clone as 18996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 18996-1-AP (same clone as 18996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18996-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

OCT2-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 18996-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

OCT2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Oct-2, Octamer-binding protein 2

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Product Information

18996-1-PBS targets OCT2-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: POU class 2 homeobox 2
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 52-66 kDa
• GenBank Accession Number: NM_002698
• Gene Symbol: POU2F2
• Gene ID (NCBI): 5452
• RRID: AB_10596624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09086
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

OCT2, also named as POU2F2 and OTF2, belongs to the POU transcription factor family and Class-2 subfamily. OCT2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). OCT2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. The antibody is specific to OCT2. OCT2 can be detected 52-66 kDa (PMID: 15377492, PMID: 34010659).