OCT2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

OCT2-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18996-1-AP

Synonyms

OCT2, OCT2-Specific, Octamer binding protein 2, OTF 2, OTF2, POU class 2 homeobox 2, POU2F2

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Validation Data Gallery

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  WB analysis of mouse thymus using 18996-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18996-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18996-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 18996-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 18996-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 18996-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18996-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse thymus tissue, human brain tissue, human spleen tissue, mouse kidney tissue, Raji cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

18996-1-AP targets OCT2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: POU class 2 homeobox 2
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 52-66 kDa
• GenBank Accession Number: NM_002698
• Gene Symbol: POU2F2
• Gene ID (NCBI): 5452
• RRID: AB_10596624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OCT2, also named as POU2F2 and OTF2, belongs to the POU transcription factor family and Class-2 subfamily. OCT2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). OCT2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. The antibody is specific to OCT2. OCT2 can be detected 52-66 kDa (PMID: 15377492, PMID: 34010659).

Publications

Species	Application	Title
human	WB	Mol Ther; miR-3687 Overexpression Promotes Bladder Cancer Cell Growth by Inhibiting the Negative Effect of FOXP1 on Cyclin E2 Transcription.; Authors - Qipeng Xie
human,mouse	WB,IHC	Int J Oncol; Receptor for activated C kinase 1 promotes cervical cancer lymph node metastasis via the glycolysis‑dependent AKT/mTOR signaling.; Authors - Lixiu Xu
human	WB	Biochem Biophys Res Commun; CBX7 represses the POU2F2 to inhibit the PD-L1 expression and regulate the immune response in bladder cancer.; Authors - Weigang Ren ; KD Validated
	WB	Toxicol In Vitro; Comparisons of segment-specific toxicity of platinum-based agents and cadmium using S1, S2, and S3 cells derived from mouse kidney proximal tubules.; Authors - Hitomi Fujishiro
mouse	WB	Age (Dordr); Aging increases the susceptibility of cisplatin-induced nephrotoxicity.; Authors - Jiagen Wen