OCT4 Monoclonal antibody, PBS Only

OCT4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C4B6

Cat no : 60242-1-PBS

Synonyms

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

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Validation Data Gallery

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  WB analysis using 60242-1-Ig (same clone as 60242-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60242-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60242-1-Ig (same clone as 60242-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60242-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 60242-1-Ig (same clone as 60242-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60242-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 60242-1-Ig (same clone as 60242-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60242-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60242-1-Ig (same clone as 60242-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60242-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 60242-1-Ig (same clone as 60242-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60242-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60242-1-Ig (same clone as 60242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60242-1-Ig (OCT4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60242-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60242-1-Ig (same clone as 60242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60242-1-Ig (OCT4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60242-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60242-1-Ig (same clone as 60242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60242-1-Ig (OCT4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60242-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60242-1-Ig (same clone as 60242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60242-1-Ig (OCT4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60242-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60242-1-PBS targets OCT4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: OCT4 fusion protein Ag1794
• Full Name: POU class 5 homeobox 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 50-52 kDa
• GenBank Accession Number: BC020712
• Gene Symbol: POU5F1
• Gene ID (NCBI): 5460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5'-ATGCAAAT-3'. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

 What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

 What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing - OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

 What is OCT4's involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).