Name: OCT4 Mouse Monoclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IHC, ELISA
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OCT4 Antibody WB HEK-293 cells 60242-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

OCT4 Antibody IHC human breast cancer tissue 60242-1-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60242-1-Ig (OCT4 antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:

human, mouse

Positive WB detected in:

HEK-293 cells, human brain tissue, NCCIT cell, Neuro-2a cells

Positive IHC detected in:

human breast cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:5000-1:20000

IHC : 1:250-1:1000

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Mouse

Purification method:

Protein G purification

Isotype:

IgG1

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

OCT4 fusion protein Ag1794

Full name:

POU class 5 homeobox 1

Calculated molecular weight:

39 kDa

Observed molecular weight:

50-52 kDa

GenBank accession number:

BC020712

Gene ID (NCBI):

5460

Gene symbol

POU5F1

Synonyms

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

Background

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5’-ATGCAAAT-3’. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing – OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

What is OCT4’s involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).