OGG1 Polyclonal antibody

OGG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

29

Reviews (3)

Cat no : 15125-1-AP

Synonyms

Ogg 1, N-glycosylase/DNA lyase, DNA-(apurinic or apyrimidinic site) lyase, 8-oxoguanine DNA glycosylase, 8 oxoguanine DNA glycosylase

---

Validation Data Gallery

• 

  WB analysis using 15125-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15125-1-AP (OGG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 15125-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15125-1-AP (OGG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 15125-1-AP

  IP result of anti-OGG1 (IP:15125-1-AP, 4ug; Detection:15125-1-AP 1:500) with mouse heart tissue lysate 3200ug.

• 

  IHC staining of mouse liver using 15125-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15125-1-AP (OGG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15125-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15125-1-AP (OGG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 15125-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using OGG1 antibody (15125-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: A549 cells, Transfected HEK-293 cells, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 25 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

15125-1-AP targets OGG1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OGG1 fusion protein Ag7204
• Full Name: 8-oxoguanine DNA glycosylase
• Calculated Molecular Weight: 22 kDa, 36-40 kDa, 45-57 kDa
• Observed Molecular Weight: 36-40 kDa, 45-50 kDa
• GenBank Accession Number: BC000657
• Gene Symbol: OGG1
• Gene ID (NCBI): 4968
• RRID: AB_2156780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The DNA damages induced by ROS contain base modification, base loss, and DNA single strand breaks, which are usually repaired by the base excision repair (BER) pathway in both prokaryotes and eukaryotes. OGG1 (The human 8-oxoguanine glycosylase 1) is the primary enzyme in BER pathway, responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct that occurs as a result of exposure to reactive oxygen species. There's 8 isoforms of OGG1, with calculated MW 22 kDa, 36-40 kDa and 45-57 kDa. The difference among these isoforms is the C-terminal (317-345aa). Our OGG1 antibody detects all the isoforms. We always got the strongest 47 kDa corresponds to isoform Beta in our detection and some weaker bands (with long time exposure). The expression amount of Beta is higher than other isoforms from our data. This antibody has been cited in more than 4 publications, WB and IHC detection in mouse and human.

Publications

Species	Application	Title
mouse	IHC	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
rat	WB	Nat Commun; Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.; Authors - Megha Jhanji
mouse	WB	PLoS Genet; Heterozygosity for a hypomorphic polβ mutation reduces the expansion frequency in a mouse model of the fragile x-related disorders.; Authors - Rachel Adihe Lokanga
mouse	WB	JHEP Rep; TAK1 deficiency promotes liver injury and tumorigenesis via ferroptosis and macrophage cGAS-STING signalling; Authors - Wantong Su
mouse	WB	Redox Biol; Role of WW domain E3 ubiquitin protein ligase 2 in modulating ubiquitination and Degradation of Septin4 in oxidative stress endothelial injury.; Authors - Naijin Zhang
mouse	WB	Hum Mol Genet; X inactivation plays a major role in the gender bias in somatic expansion in a mouse model of the fragile X-related disorders: implications for the mechanism of repeat expansion.; Authors - Rachel Adihe Lokanga

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Shubhangi (Verified Customer) (07-05-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: Ac16

OGG1 Antibody Western Blot validation (1000 dilution) in Ac16 (Cat no:15125-1-AP)

FH

s (Verified Customer) (12-12-2022)

very good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mouse lung

OGG1 Antibody Western Blot validation (1000 dilution) in mouse lung (Cat no:15125-1-AP)

FH

Ruyi (Verified Customer) (08-19-2019)

This antibody works well in my WB experiments.

• Applications: Western Blot,