OGT Monoclonal antibody

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  WB analysis using 66823-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66823-1-Ig (OGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66823-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66823-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66823-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66823-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66823-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGT antibody (66823-1-Ig, Clone: 2B2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

OGT Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66823-1-Ig

Clone No.2B2A6

Publications(9)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FLJ23071, HRNT1, OGT

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

66823-1-Ig targets OGT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28402

  Product name: Recombinant human OGT protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-400 aa of BC014434

  Sequence: MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQD

• Full Name: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• Calculated Molecular Weight: 1046 aa, 117 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC014434
• Gene Symbol: OGT
• Gene ID (NCBI): 8473
• RRID: AB_2882166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15294
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Adv Sci (Weinh); LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease; Authors - Fuji Yang
human	WB	Sci Adv; FOXA1 O-GlcNAcylation-mediated transcriptional switch governs metastasis capacity in breast cancer; Authors - Yajie Liu ; KD Validated
human	WB,IHC	Mol Cell Biochem; O-GlcNAcylation of hexokinase 2 modulates mitochondrial dynamics and enhances the progression of lung cancer; Authors - S I Panpan
human	WB	J Biol Chem; Histone methyltransferase Dot1L recruits O-GlcNAc transferase to target chromatin sites to regulate histone O-GlcNAcylation.; Authors - Bo Xu
human	WB	World J Gastrointest Oncol; Senegenin suppresses hepatocellular carcinoma by regulating O-GlcNAcylation; Authors - Xiang Zhang
human,mouse	WB,IHC	Oncogene; Chromatin-associated OGT promotes the malignant progression of hepatocellular carcinoma by activating ZNF263; Authors - Lingyan Wang ; KD Validated