CoraLite® Plus 488-conjugated OGT Monoclonal antibody

• 

  WB analysis using CL488-66823

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66823 (OGT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

OGT Monoclonal Antibody for WB

Cat No. CL488-66823

Clone No.2B2A6

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB

Synonyms

2B2A6, EC:2.4.1.255, HRNT1, O GLCNAC, O-GlcNAc transferase subunit p110

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66823 targets OGT in WB applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28402

  Product name: Recombinant human OGT protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-400 aa of BC014434

  Sequence: MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQD

• Full Name: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• Calculated Molecular Weight: 1046 aa, 117 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC014434
• Gene Symbol: OGT
• Gene ID (NCBI): 8473
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15294
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.