OPA1 Polyclonal antibody

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• OPA1 Polyclonal antibody (27733-1-AP)

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• KD/KO Validated

OPA1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 27733-1-AP Copied!

Publications (323)

Reviews (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Synonyms

Dynamin-like GTPase OPA1, long form, Dynamin-like GTPase OPA1, mitochondrial, Dynamin-like GTPase OPA1, short form, EC:3.6.5.5, OPA 1

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  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HEK-293 using 27733-1-AP

  WB result of OPA1 antibody (27733-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPA1 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 27733-1-AP

  IP result of anti-OPA1 (IP:27733-1-AP, 4ug; Detection:27733-1-AP 1:10000) with mouse brain tissue lysate 1320 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse heart using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 27733-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug OPA1 Polyclonal antibody (27733-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, mouse brain tissue, HeLa cells, HepG2 cells, rat brain tissue, HEK-293T cells, L02 cells, mouse liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27733-1-AP targets OPA1 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, hamster, goat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26887

  Product name: Recombinant human OPA1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 607-960 aa of BC075805

  Sequence: SLSQVTPKHWEEILQQSLWERVSTHVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEEALQARLKDTENAIENMVGPDWKKRWLYWKNRTQEQCVHNETKNELEKMLKCNEEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTALNHCNLCRRGFYYYQRHFVDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNVKEVLEDFAEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK

• Full Name: optic atrophy 1 (autosomal dominant)
• Calculated Molecular Weight: 960 aa, 112 kDa
• Observed Molecular Weight: 80-100 kDa
• GenBank Accession Number: BC075805
• Gene Symbol: OPA1
• Gene ID (NCBI): 4976
• RRID: AB_2810292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60313
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.

Publications

What published studies show

The anti-DRP1 antibody (Cat# 27733-1-AP) has accumulated 323 total citations across high-impact journals including Nature, Science, Cell Metabolism, Nature Communications, Molecular Cell, Cell Death & Differentiation, Free Radical Biology and Medicine, and International Journal of Biological Sciences. Research fields span mitochondrial dynamics (fission/fusion), neurodegeneration (Parkinson's, Alzheimer's, ALS), cardiovascular disease, cancer, metabolic disorders (diabetes, obesity), renal disease, oxidative stress, ferroptosis, stroke, inflammation, and environmental toxicology.

Automatically generated from the 323 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Lysosomes drive the piecemeal removal of mitochondrial inner membrane; Authors - Akriti Prashar
mouse	WB	Nature; Dual regulation of mitochondrial fusion by Parkin-PINK1 and OMA1; Authors - Tatsuya Yamada
human	WB	Science; Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function; Authors - Wonyul Jang
mouse,human	WB	Cell Metab; Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation; Authors - Jian Zhou
human	CoIP	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nat Cell Biol; A transport-independent role for SLC25A12 in mitochondrial stress signalling; Authors - Liu Jiang

Reviews

5.0 out of 5

9 verified customer reviews

What customers say

All 9 reviews rate this antibody 5 stars. Users consistently report excellent performance, primarily in Western Blot, with clean bands at expected molecular weights across diverse cell types including HeLa, HEK, corneal cells, adipocytes, and primary cells. Several reviewers also confirmed successful use in IHC, IF, IP, and flow cytometry. Recommended dilutions range from 1:500 to 1:5,000. One user noted the absence of a second band in their specific application but found overall results as expected. The antibody is described as easy to use and reliable for reproducible results.

Automatically generated from the 9 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Chun (Verified Customer) (12-22-2025)

This is an excellent antibody for Immunoblotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5,000
• Cell Tissue Type: HeLa cells

Dhanwini (Verified Customer) (09-24-2025)

GOOD

• Applications: Western Blot

Robert (Verified Customer) (09-24-2025)

Didn't get two bands in my application, but otherwise turned out as expected

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCEC, HCET

Sneha (Verified Customer) (09-24-2025)

GOOD

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ARPE-19

Michael (Verified Customer) (09-23-2025)

Excellent

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Corneal cells

Henry (Verified Customer) (09-23-2025)

Ease of use

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1:4500
• Cell Tissue Type: MIOM1 PBMCS

Kahimbi (Verified Customer) (01-31-2025)

It was used as a primary antibody for a western blot and the bands were clearly visible. The antibody worked well.

• Applications: Western Blot

P (Verified Customer) (09-23-2024)

excellent!

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEK

OPA1 Antibody Western Blot validation (1000 dilution) in HEK (Cat no:27733-1-AP)

Mi (Verified Customer) (02-21-2023)

Works well in human adipocyte cells, we got clean bands at the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes