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  • KD/KO Validated

OPA3 Polyclonal antibody

OPA3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15638-1-AP

Synonyms

MGA3, OPA3, Optic atrophy 3 protein



Tested Applications

Positive WB detected inA549 cells, human kidney tissue, mouse thymus tissue, Jurkat cells, HeLa cells, HEK-293T cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15638-1-AP targets OPA3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPA3 fusion protein Ag8103
Full Name optic atrophy 3 (autosomal recessive, with chorea and spastic paraplegia)
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC005059
Gene Symbol OPA3
Gene ID (NCBI) 80207
RRIDAB_2158168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The OPA3 cDNA encodes a deduced 179-amino acid protein. Northern blot analysis demonstrated a primary transcript of approximately 5.0 kb that was ubiquitously expressed, most prominently in skeletal muscle and kidney. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract.

Protocols

Product Specific Protocols
WB protocol for OPA3 antibody 15638-1-APDownload protocol
IHC protocol for OPA3 antibody 15638-1-APDownload protocol
IF protocol for OPA3 antibody 15638-1-APDownload protocol
IP protocol for OPA3 antibody 15638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Hum Mol Genet

Disrupted mitochondrial function in the Opa3L122P mouse model for Costeff Syndrome impairs skeletal integrity.

Authors - Alice E Navein
mouse,ratWB,IP

Cell Signal

Hydrogen sulfide alleviates mitochondrial damage and ferroptosis by regulating OPA3-NFS1 axis in doxorubicin-induced cardiotoxicity

Authors - Yifan Wang
human,mouseWB,IHC

Invest Ophthalmol Vis Sci

Mitochondrial localization and ocular expression of mutant Opa3 in a mouse model of 3-methylglutaconicaciduria type III.

Authors - Powell Kate A KA
bovineWB

Genomics

A nonsense mutation in the optic atrophy 3 gene (OPA3) causes dilated cardiomyopathy in Red Holstein cattle.

Authors - Owczarek-Lipska Marta M
humanWB

Bioengineered

MYB proto-oncogene like 2 promotes hepatocellular carcinoma growth and glycolysis via binding to the Optic atrophy 3 promoter and activating its expression.

Authors - Miao Liu
  • KD Validated
humanIHC

Cancers (Basel)

Oncogenic K-ras Induces Mitochondrial OPA3 Expression to Promote Energy Metabolism in Pancreatic Cancer Cells.

Authors - Ning Meng