OPN4 Polyclonal antibody

OPN4 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, ELISA

Conjugate

Unconjugated

Cat no : 24478-1-AP

Synonyms

Melanopsin, MOP, OPN4, opsin 4

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Validation Data Gallery

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  IF Staining of mouse eye using 24478-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OPN4 antibody (24478-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IF detected in: mouse eye tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24478-1-AP targets OPN4 in IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OPN4 fusion protein Ag21643
• Full Name: opsin 4
• Calculated Molecular Weight: 478 aa, 53 kDa
• GenBank Accession Number: BC113558
• Gene Symbol: OPN4
• Gene ID (NCBI): 94233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPN4, an opsin that was first isolated from the skin of Xenopus laevis in 1998, belongs not to the vertebrate-type opsin family but rather to the invertebrate-type opsins, which are part of the seven-transmembrane G protein-coupled receptor (GPCR) family (PMID:31989708). In humans, OPN4 is expressed in the retinal ganglion cells in addition to part of the brain (PMID:10632589). Moreover, Opn4 functions as a photoreceptor by detecting brightness levels and discriminating visual signals (PMID:22633808). Opn4 also facilitates visual circuits to acquire optimal settings and light adaptation by measuring irradiance levels (PMID:25517373).