ORAI3 Polyclonal antibody

ORAI3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 25766-1-AP

Synonyms

ORAI3, Protein orai 3, TMEM142C, Transmembrane protein 142C

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Validation Data Gallery

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  WB analysis of mouse heart using 25766-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25766-1-AP (ORAI3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human endometrial cancer using 25766-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25766-1-AP (ORAI3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 25766-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25766-1-AP (ORAI3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 25766-1-AP is ORAI3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ORAI3 fusion protein Ag22513
• Full Name: ORAI calcium release-activated calcium modulator 3
• Calculated molecular weight: 295 aa, 32 kDa
• Observed molecular weight: ~30 kDa
• GenBank accession number: BC006126
• Gene symbol: ORAI3
• Gene ID (NCBI): 93129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ORAI3, also named as TMEM142C, is a key regulator or component of store-operated Ca2+ channel and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to the process of Ca2+ depotentiation. The MW of ORAI3 is about 30-32 kDa.

Publications

Species	Application	Title
human	WB,IF,CoIP	BMJ Open Diabetes Res Care; Orai-IGFBP3 signaling complex regulates high-glucose exposure-induced increased proliferation, permeability, and migration of human coronary artery endothelial cells.; Authors - Suwen Bai
mouse	IP	BMJ Open Diabetes Res Care; Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.; Authors - Yuan Wei
human	WB	Exp Ther Med; Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.; Authors - Wu Lele

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (02-06-2023)

Works pretty well for our WB

• Applications: Western Blot