OSBPL11 Polyclonal antibody

OSBPL11 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11318-1-AP

Synonyms

ORP 11, ORP11, OSBP related protein 11, OSBP12, OSBPL11, TCCCIA00292

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Validation Data Gallery

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  WB analysis using 11318-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11318-1-AP (OSBPL11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11318-1-AP

  IP result of anti-OSBPL11 (IP:11318-1-AP, 4ug; Detection:11318-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 11318-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11318-1-AP (OSBPL11 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11318-1-AP targets OSBPL11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSBPL11 fusion protein Ag1855
• Full Name: oxysterol binding protein-like 11
• Calculated Molecular Weight: 747 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC010422
• Gene Symbol: OSBPL11
• Gene ID (NCBI): 114885
• RRID: AB_2267735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		Elife; The ORP9-ORP11 dimer promotes sphingomyelin synthesis; Authors - Birol Cabukusta