Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human ovary cancer tissue, rat ovary tissue, mouse ovary tissue, mouse liver tissue, human placenta tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11318-1-AP targets OSBPL11 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1855 Product name: Recombinant human OSBPL11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-367 aa of BC010422 Sequence: MDLTRVVLPTFILEKRSLLEMYADFMSHPDLFIAITNGATAEDRMIRFVEYYLTSFHEGRKGAIAKKPYNPIIGETFHCSWKMPKSEVASSVFSSSSTQGVTNHAPLSGESLTQVGSDCYTVRFVAEQVSHHPPVSGFYAECTERKMCVNAHVWTKSKFLGMSIGVTMVGEGILSLLEHGEEYTFSLPCAYARSILTVPWVELGGKVSVNCAKTGYSASITFHTKPFYGGKLHRVTAEVKHNITNTVVCRVQGEWNSVLEFTYSNGETKYVDLTKLAVTKKRVRPLEKQDPFESRRLWKNVTDSLRESEIDKATEHKHTLEERQRTEERHRTETGTPWKTKYFIKEGDGWVYHKPLWKIIPTTQPAE Predict reactive species |
| Full Name | oxysterol binding protein-like 11 |
| Calculated Molecular Weight | 747 aa, 84 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC010422 |
| Gene Symbol | OSBPL11 |
| Gene ID (NCBI) | 114885 |
| RRID | AB_2267735 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXB4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for OSBPL11 antibody 11318-1-AP | Download protocol |
| IP protocol for OSBPL11 antibody 11318-1-AP | Download protocol |
| WB protocol for OSBPL11 antibody 11318-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The 11318-1-AP OSBPL11 Polyclonal antibody has 4 total citations. These appear in Autophagy, Journal of Neuroinflammation, Biochemical and Biophysical Research Communications, and eLife. The associated research fields include lysosomal membrane repair and neuronal lipid homeostasis after spinal cord injury, neuronal ferroptosis, lipid-transport activity and mitotic slippage in cancer drug cytotoxicity, and sphingomyelin synthesis. Applications cited include Western blot and immunofluorescence in mouse and human samples.
| Species | Application | Title |
|---|---|---|
Autophagy The PI4K2A-OSBPL6/ORP6-PS axis mediates lysosomal membrane repair to restore neuronal lipid homeostasis and promote neuronal survival after spinal cord injury. | ||
J Neuroinflammation OSBPL10 alleviates neuronal ferroptosis via lysosomal membrane repair in a PS-dependent manner after spinal cord injury. | ||
Biochem Biophys Res Commun Suppression of the lipid-transport activity of OSBPL11 weakens the cytotoxicity of SAC-activating drugs by promoting mitotic slippage.
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