Filter: AllWBIHC

WB analysis using 32158-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 32158-1-AP (OSBPL6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of mouse skeletal muscle using 32158-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 32158-1-AP (OSBPL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 32158-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 32158-1-AP (OSBPL6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 32158-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32158-1-AP (OSBPL6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 32158-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32158-1-AP (OSBPL6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OSBPL6 Polyclonal antibody

Name: OSBPL6 Polyclonal antibody
Brand: Proteintech
SKU: 32158-1-AP

OSBPL6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32158-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ORP6, ORP-6, OSBP-related protein 6, Oxysterol-binding protein-related protein 6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	HEL cells
Positive IHC detected in	mouse skeletal muscle tissue, mouse brain tissue, mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

32158-1-AP targets OSBPL6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag37534 Product name: Recombinant human OSBPL6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 189-308 aa of BC052259 Sequence: RSPRDASFHIFPSTSTAESSPAANVSVMDGKMQPNSFPWQSPLPCSNSLPATCTTGQSKVAAWLQDSEEMDRCAEDLAHCQSNLVELSKLLQNLEILQRTQSAPNFTDMQVPFSATMSPV Predict reactive species
Full Name	oxysterol binding protein-like 6
Calculated Molecular Weight	934 aa, 106 kDa
Observed Molecular Weight	106 kDa
GenBank Accession Number	BC052259
Gene Symbol	OSBPL6
Gene ID (NCBI)	114880
RRID	AB_3742533
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q9BZF3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.