P21 Polyclonal antibody

P21 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 27296-1-AP

Synonyms

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, PIC1, SDI1, WAF1



Tested Applications

Positive WB detected inNeuro-2a cells, C6 cells, NIH/3T3 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 7 publications below

Product Information

The immunogen of 27296-1-AP is P21 Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P21 fusion protein Ag26129
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC000275
Gene symbol CDKN1A
Gene ID (NCBI) 1026
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Protocols

Product Specific Protocols
WB protocol for P21 antibody 27296-1-APDownload protocol
IF protocol for P21 antibody 27296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.

Authors - Yanzhuo Zhang
mouseWB

Exp Ther Med

MicroRNA-22 contributes to dexamethasone-induced osteoblast differentiation inhibition and dysfunction through targeting caveolin-3 expression in osteoblastic cells.

Authors - Peng Li
humanWB

PeerJ

Involvement of plasminogen activator inhibitor-1 and its related molecules in atrial fibrosis in patients with atrial fibrillation.

Authors - Qiaoqiao Li
mouseWB

Int J Mol Med

The DR1‑CSE/H2S system inhibits renal fibrosis by downregulating the ERK1/2 signaling pathway in diabetic mice.

Authors - Hongzhu Li
humanWB

Int J Oncol

PMID: 32319562

Authors - Rui Chen
mouseWB

Lab Invest

Th17/IL-17 induces endothelial cell senescence via activation of NF-κB/p53/Rb signaling pathway.

Authors - Liang Zhang