P3H1 Polyclonal antibody

P3H1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26691-1-AP

Synonyms

GROS1, Growth suppressor 1, LEPRE1, Leprecan 1, P3H1, Prolyl 3 hydroxylase 1

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Validation Data Gallery

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  WB analysis of HeLa using 26691-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26691-1-AP (P3H1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 26691-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26691-1-AP (P3H1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 26691-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26691-1-AP (P3H1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 26691-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26691-1-AP (P3H1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 26691-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26691-1-AP (P3H1 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 26691-1-AP is P3H1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P3H1 fusion protein Ag24904
• Full Name: leucine proline-enriched proteoglycan (leprecan) 1
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC108311
• Gene Symbol: LEPRE1/P3H1
• Gene ID (NCBI): 64175
• RRID: AB_2880605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

P3H1 (prolyl 3-hydroxylase 1, also known as LEPRE1) is a member of the leprecan family of proteins, which also include P3H2, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H1 is localized to the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. Mutations in this gene are associated with osteogenesis imperfecta type VIII.

Publications

Species	Application	Title
human	WB,IHC	J Oncol; The Prognostic Significance and Potential Mechanism of Prolyl 3-Hydroxylase 1 in Hepatocellular Carcinoma; Authors - Chunlei Li
	WB	Acta Biomater; Matrix produced by diseased cardiac fibroblasts affects early myotube formation and function; Authors - Janny Piñeiro-Llanes