PAFAH2 Polyclonal antibody

PAFAH2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10085-1-AP

Synonyms

HSD PLA2, PAFAH2, SD PLA2



Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10085-1-AP targets PAFAH2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAFAH2 fusion protein Ag0124
Full Name platelet-activating factor acetylhydrolase 2, 40kDa
Calculated molecular weight 40 kDa
Observed molecular weight 44-46 kDa
GenBank accession numberBC001158
Gene symbol PAFAH2
Gene ID (NCBI) 5051
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The family of platelet-activating factor-acetylhydrolase (PAF-AH) consists of enzymes which catalyze the hydrolysis of acetyl ester at the sn-2 position of PAF(Platelet-activating factor). The intracellular type II PAF-AH is a single polypeptide about a 40 kd distributed in both the cytosol and membranes of many kinds of cells.

Protocols

Product Specific Protocols
WB protocol for PAFAH2 antibody 10085-1-APDownload protocol
IHC protocol for PAFAH2 antibody 10085-1-APDownload protocol
IP protocol for PAFAH2 antibody 10085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Biochemistry

Trafficking of platelet-activating factor acetylhydrolase type II in response to oxidative stress.

Authors - Thévenin Anastasia F AF
humanWB

J Lipid Res

Intracellular PAF catabolism by PAF acetylhydrolase counteracts continual PAF synthesis.

Authors - Chen Jiawei J
humanWB

J Biol Chem

Oxidatively truncated phospholipids are required agents of tumor necrosis factor α (TNFα)-induced apoptosis.

Authors - Latchoumycandane Calivarathan C