|Positive WB detected in||mouse liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10085-1-AP targets PAFAH2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PAFAH2 fusion protein Ag0124|
|Full Name||platelet-activating factor acetylhydrolase 2, 40kDa|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||44-46 kDa|
|GenBank accession number||BC001158|
|Gene ID (NCBI)||5051|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The family of platelet-activating factor-acetylhydrolase (PAF-AH) consists of enzymes which catalyze the hydrolysis of acetyl ester at the sn-2 position of PAF(Platelet-activating factor). The intracellular type II PAF-AH is a single polypeptide about a 40 kd distributed in both the cytosol and membranes of many kinds of cells.
Trafficking of platelet-activating factor acetylhydrolase type II in response to oxidative stress.
J Lipid Res
Intracellular PAF catabolism by PAF acetylhydrolase counteracts continual PAF synthesis.
J Biol Chem
Oxidatively truncated phospholipids are required agents of tumor necrosis factor α (TNFα)-induced apoptosis.