PAIP1 Polyclonal antibody

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PAIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 10675-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

PABP interacting protein 1, PABP-interacting protein 1, PAIP 1, PAIP-1, Poly(A)-binding protein-interacting protein 1

• 

  WB analysis of HeLa using 10675-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10675-1-AP

  WB result of PAIP1 antibody (10675-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAIP1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COS-7 using 10675-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of BxPC-3 using 10675-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of BxPC-3 using 10675-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 10675-1-AP

  IP result of anti-PAIP1 (IP:10675-1-AP, 4ug; Detection:10675-1-AP 1:300) with HeLa cells lysate 3000 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 10675-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PAIP1 antibody (10675-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, BxPC-3 cells, COS-7 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10675-1-AP targets PAIP1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1070

  Product name: Recombinant human PAIP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 102-400 aa of BC005295

  Sequence: EQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ

• Full Name: poly(A) binding protein interacting protein 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 60 kDa, 51 kDa, 40 kDa
• GenBank Accession Number: BC005295
• Gene Symbol: PAIP1
• Gene ID (NCBI): 10605
• RRID: AB_2160391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H074
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545].

Publications

What published studies show

The PAIP1 antibody (Cat# 10675-1-AP) has 7 total citations. These appear in journals including Cancer Research and Treatment, BMC Genomics, Biochemical Journal, Experimental Cell Research, Archives of Biochemistry and Biophysics, Biology of Reproduction, and Human Pathology. The research fields span cervical cancer prognosis, alternative splicing regulation of cancer genes, translation initiation factor expression, pancreatic cancer angiogenesis, hepatocellular carcinoma proliferation, spermiogenic mRNA translation, and lung adenocarcinoma tumor progression via the AKT/GSK-3β pathway.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Cancer Res Treat; Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis.; Authors - Nan Li ; KD Validated
human	WB	Biochem J; Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity.; Authors - Emma Jane Mead
human	RIP,CoIP	BMC Genomics; PAIP1 binds to pre-mRNA and regulates alternative splicing of cancer pathway genes including VEGFA; Authors - Jianfeng Zheng
human	WB,IF	Exp Cell Res; Role of Paip1 on angiogenesis and invasion in pancreatic cancer.; Authors - Hongjian Guan ; KD Validated
human	WB	Arch Biochem Biophys; YAP1-activated ZNF131 promotes hepatocellular carcinoma cell proliferation through transcriptional regulation of PAIP1; Authors - Guozhi Yin
human	WB,IHC,IF	Biol Reprod; Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2.; Authors - Yue He