PAK1 Polyclonal antibody
PAK1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 51137-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, A431 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Product Information
51137-1-AP targets PAK1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | p21 protein (Cdc42/Rac)-activated kinase 1 |
Calculated molecular weight | 553 aa, 62 kDa |
Observed molecular weight | 61-65 kDa |
GenBank accession number | BC109299 |
Gene symbol | PAK1 |
Gene ID (NCBI) | 5058 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for PAK1 antibody 51137-1-AP | Download protocol |
IHC protocol for PAK1 antibody 51137-1-AP | Download protocol |
IF protocol for PAK1 antibody 51137-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |