PAK1 Polyclonal antibody

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  WB analysis using 51137-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 51137-1-AP

  WB result of PAK1 antibody (51137-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK1 transfected HEK-293 cells.

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  WB analysis of K-562 using 51137-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 51137-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 51137-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 51137-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 51137-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK1 antibody (51137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

PAK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 51137-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Alpha PAK, p21 activated kinase 1, p65 PAK, PAK 1, PAK1, PAKalpha

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Tested Applications

• Positive WB detected in: A431 cells, K-562 cells, HEK-293 cells, C6 cells, NIH/3T3 cells, PC-12 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

51137-1-AP targets PAK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: p21 protein (Cdc42/Rac)-activated kinase 1
• Calculated Molecular Weight: 553 aa, 62 kDa
• Observed Molecular Weight: 61-65 kDa
• GenBank Accession Number: BC109299
• Gene Symbol: PAK1
• Gene ID (NCBI): 5058
• RRID: AB_2283523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13153
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.

Publications

Species	Application	Title
human	IHC	Pathol Res Pract; Clinicopathological significance and the associated signaling pathway of p21-activated kinase 1 (PAK1) in colorectal cancer; Authors - Jung-Soo Pyo
human	WB	Commun Biol; Excessive endometrial PlGF- Rac1 signalling underlies endometrial cell stiffness linked to pre-eclampsia; Authors - Janet P Raja Xavier
human,mouse	WB	J Environ Manage; Exosomal miR-129-2-3p promotes airway epithelial barrier disruption in PM2.5-aggravated asthma; Authors - Caihong Wang