Palladin-Specific Polyclonal antibody

Palladin-Specific Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 16179-1-AP

Synonyms

CGI 151, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

16179-1-AP targets Palladin-Specific in WB, IP,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name palladin, cytoskeletal associated protein
Calculated molecular weight 151 kDa
Observed molecular weight 120 kDa
GenBank accession numberNM_016081
Gene symbol PALLD
Gene ID (NCBI) 23022
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Palladin is a widely expressed protein found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin. It is a cytoskeletal protein required for organization of normal actin cytoskeleton. It functions in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. This antibody, generated against N-terminal peptide of palladin, recognizes 200 kDa, 140 kDa, and 65 kDa isoforms of this protein.

Protocols

Product Specific Protocols
WB protocol for Palladin-Specific antibody 16179-1-APDownload protocol
IP protocol for Palladin-Specific antibody 16179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols