PANK4 Polyclonal antibody

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  WB analysis using 32953-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32953-1-AP (PANK4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

PANK4 Polyclonal Antibody for WB, ELISA

Cat No. 32953-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

4'-phosphopantetheine phosphatase, EC:3.1.3.-, hPanK4, Inactive pantothenic acid kinase 4

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32953-1-AP targets PANK4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38232

  Product name: Recombinant human PANK4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-178 aa of NM_018216.2

  Sequence: MAECGASGSGSSGDSLDKSITLPPDEIFRNLENAKRFAIDIGGSLTKLAYYSTVQHKVAKVRSFDHSGKDTEREHEPPYEISVQEEITARLHFIKFENTYIEACLDFIKDHLVNTETKVIQATGGGAYKFKDLIEEKLRLKVDKEDVMTCLIKGCNFVLKNIPHEAFVYQKDSDPEFR

• Full Name: pantothenate kinase 4
• Calculated Molecular Weight: 86 kDa，773aa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: NM_018216.2
• Gene Symbol: PANK4
• Gene ID (NCBI): 55229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NVE7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PANK4 (Pantothenate Kinase 4) is a member of the pantothenate kinase family, which is involved in the biosynthesis of coenzyme A (CoA). However, unlike other members of the family, PANK4 lacks the residues required for kinase activity and instead contains a phosphatase domain (DUF89) that can dephosphorylate 4'-phosphopantetheine, an intermediate in CoA biosynthesis (PMID: 30927326). This suggests that PANK4 may act as a pseudoenzyme, regulating cellular CoA levels by antagonizing CoA production (PMID: 39746949).