Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21631-1-AP targets PATL1 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16247 Product name: Recombinant human PATL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 278-627 aa of BC109038 Sequence: LQSTDPYLDDFYYQNYFEKLEKLSAAEEIQGDGPKKERTKLITPQVAKLEHAYKPVQFEGSLGKLTVSSVNNPRKMIDAVVTSRSEDDETKEKQVRDKRRKTLVIIEKTYSLLLDVEDYERRYLLSLEEERPALMDDRKHKICSMYDNLRGKLPGQERPSDDHFVQIMCIRKGKRMVARILPFLSTEQAADILMTTARNLPFLIKKDAQDEVLPCLLSPFSLLLYHLPSVSITSLLRQLMNLPQSAATPALSNPHLTAVLQNKFGLSLLLILLSRGEDLQSSDPATESTQNNQWTEVMFMATRELLRIPQAALAKPISIPTNLVSLFSRYVDRQKLNLLETKLQLVQGIR Predict reactive species |
| Full Name | protein associated with topoisomerase II homolog 1 (yeast) |
| Calculated Molecular Weight | 770 aa, 87 kDa |
| Observed Molecular Weight | 87 kDa |
| GenBank Accession Number | BC109038 |
| Gene Symbol | PATL1 |
| Gene ID (NCBI) | 219988 |
| RRID | AB_2935454 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86TB9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Patellin1 (PATL1) is a human homolog to yeast P body component Pat1, localizes to P bodies and is required for P body formation. Furthermore, Pat1 is the only decapping activator protein that, besides interacting with deadenylated mRNAs, also binds to eIF4G-, eIF4E- and Pab1p-associated polyadenylated mRNA and locates to polysomes.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for PATL1 antibody 21631-1-AP | Download protocol |
| WB protocol for PATL1 antibody 21631-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PATL1 Polyclonal antibody (Cat# 21631-1-AP) has 1 citation. It appears in Clinical and Translational Medicine (IF 7.9, 2025), used for Western blot in human samples. The cited study investigates FTO-mediated m6A demethylation regulating IGFBP3 expression and AKT activation via IMP3-dependent P-body re-localisation in lung cancer, placing the research in the fields of RNA modification, P-body biology, and lung oncology.
| Species | Application | Title |
|---|---|---|
Clin Transl Med FTO-mediated m6A demethylation regulates IGFBP3 expression and AKT activation through IMP3-dependent P-body re-localisation in lung cancer |
Reviews
What customers say
The product has one review, rated 4 out of 5 stars, from a Western Blot application. The reviewer described the performance as "okay," indicating acceptable but not outstanding results. No specific dilution, tissue, or cell line details were provided. Overall, the feedback suggests the antibody functions adequately for its intended use, though the reviewer did not highlight any exceptional performance or issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aditya (Verified Customer) (10-08-2024) | okay
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