PATL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PATL1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 21631-1-AP

Synonyms

PAT1 like protein 1, patellin1, PATL1, Protein PAT1 homolog 1

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Validation Data Gallery

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  WB analysis using 21631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21631-1-AP (PATL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21631-1-AP

  WB result of PATL1 antibody (21631-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PATL1 transfected HeLa cells.

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  IP experiment of K-562 using 21631-1-AP

  IP result of anti-PATL1 (IP:21631-1-AP, 4ug; Detection:21631-1-AP 1:300) with K-562 cells lysate 3320ug.

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, K-562 cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21631-1-AP targets PATL1 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PATL1 fusion protein Ag16247
• Full Name: protein associated with topoisomerase II homolog 1 (yeast)
• Calculated Molecular Weight: 770 aa, 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC109038
• Gene Symbol: PATL1
• Gene ID (NCBI): 219988
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Patellin1 (PATL1) is a human homolog to yeast P body component Pat1, localizes to P bodies and is required for P body formation. Furthermore, Pat1 is the only decapping activator protein that, besides interacting with deadenylated mRNAs, also binds to eIF4G-, eIF4E- and Pab1p-associated polyadenylated mRNA and locates to polysomes.