PCDH21 Recombinant antibody

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  WB analysis of Y79 using 84084-3-RR

  Y79 cells were subjected to SDS PAGE followed by western blot with 84084-3-RR (PCDH21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84084-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84084-3-RR against Human PCDH21 were performed. The affinity constant is 0.196 nM.

PCDH21 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84084-3-RR

Clone No.241134E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PRCAD, Photoreceptor cadherin, KIAA1775, CDHR1, Cadherin-related family member 1

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Tested Applications

• Positive WB detected in: Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84084-3-RR targets PCDH21 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PCDH21 fusion protein Ag11451
• Full Name: protocadherin 21
• Calculated Molecular Weight: 745 aa, 82 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC038799
• Gene Symbol: PCDH21
• Gene ID (NCBI): 92211
• RRID: AB_3671652
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96JP9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). PCDH21 (protocadherin 21), also known as CDHR1, is a photoreceptor-specific cadherin protein containing six ECs and an intracellular domain. The mutations of PCDH21 cause recessive retinal degeneration and autosomal recessive cone-rod dystrophy in humans (PMID: 16288196; PMID: 20805371). The full length of the PCDH21 protein is 120 kDa, the N-terminal soluble fragment is 95 kDa, and the C-terminal fragment is 25 kDa (PMID: 35066146; PMID: 18654668; PMID: 33964272).