PCDH21 Recombinant antibody, PBS Only (Capture)

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  WB analysis of Y79 using 84084-3-RR (same clone as 84084-3-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 84084-3-RR (PCDH21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84084-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00999-2

  Cytometric bead array standard curve of MP00999-2, PCDH21 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84084-3-PBS. Detection antibody: 84084-2-PBS. Standard: Ag11451. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84084-3-PBS

  Biolayer interferometry (BLl) kinetic assays of 84084-3-PBS against Human PCDH21 were performed. The affinity constant is 0.196 nM.

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  Affinity and Kinetic Characterization of 84084-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84084-3-RR against Human PCDH21 were performed. The affinity constant is 0.196 nM.

PCDH21 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 84084-3-PBS

Clone No.241134E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

241134E10, Cadherin-related family member 1, CDHR1, KIAA1775, Photoreceptor cadherin

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Product Information

84084-3-PBS targets PCDH21 as part of a matched antibody pair:

MP00999-2: 84084-3-PBS capture and 84084-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PCDH21 fusion protein Ag11451
• Full Name: protocadherin 21
• Calculated Molecular Weight: 745 aa, 82 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC038799
• Gene Symbol: PCDH21
• Gene ID (NCBI): 92211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96JP9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). PCDH21 (protocadherin 21), also known as CDHR1, is a photoreceptor-specific cadherin protein containing six ECs and an intracellular domain. The mutations of PCDH21 cause recessive retinal degeneration and autosomal recessive cone-rod dystrophy in humans (PMID: 16288196; PMID: 20805371). The full length of the PCDH21 protein is 120 kDa, the N-terminal soluble fragment is 95 kDa, and the C-terminal fragment is 25 kDa (PMID: 35066146; PMID: 18654668; PMID: 33964272).