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WB analysis using 66862-1-Ig (same clone as 66862-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66862-1-Ig (PCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66862-1-Ig (same clone as 66862-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66862-1-Ig (same clone as 66862-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66862-1-Ig (same clone as 66862-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66862-1-Ig (same clone as 66862-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66862-1-Ig (same clone as 66862-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PCK1 antibody (66862-1-Ig, Clone: 2F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

PCK1 Monoclonal antibody, PBS Only

PCK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66862-1-PBS

Clone No.2F11B5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66862-1-PBS targets PCK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity	Human, Mouse, Rat, Pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag9720 Product name: Recombinant human PCK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 274-622 aa of BC023978 Sequence: EGEKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM Predict reactive species
Full Name	phosphoenolpyruvate carboxykinase 1 (soluble)
Calculated Molecular Weight	622 aa, 69 kDa
Observed Molecular Weight	65-69 kDa
GenBank Accession Number	BC023978
Gene Symbol	PCK1
Gene ID (NCBI)	5105
RRID	AB_2882200
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P35558
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).