|Positive WB detected in||HEK-293 cells, NIH/3T3 cells, HT-1080 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human tonsillitis tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, NIH/3T3 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19856-1-AP is PCM1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PCM1 fusion protein Ag13929|
|Full Name||pericentriolar material 1|
|Calculated molecular weight||2024 aa, 228 kDa|
|Observed molecular weight||260-280 kDa|
|GenBank accession number||BC000453|
|Gene ID (NCBI)||5108|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).
|Product Specific Protocols|
|WB protocol for PCM1 antibody 19856-1-AP||Download protocol|
|IHC protocol for PCM1 antibody 19856-1-AP||Download protocol|
|IF protocol for PCM1 antibody 19856-1-AP||Download protocol|
|FC protocol for PCM1 antibody 19856-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.
CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.
Front Cell Dev Biol
Distinct Roles of TRAPPC8 and TRAPPC12 in Ciliogenesis via Their Interactions With OFD1.
Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.
J Biol Chem
The X-linked Deubiquitinase USP9X Is an Integral Component of Centrosome.
J Cell Sci
Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Julie (Verified Customer) (01-06-2020)
This antibody works great and reliably with PFA fix & methanol permeabilization.