PCM1 Polyclonal antibody

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• PCM1 Polyclonal antibody (19856-1-AP)

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PCM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19856-1-AP Copied!

Publications (36)

Reviews (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA
For FC, select conjugated version.

Synonyms

hPCM 1, hPCM-1, PCM 1, PCM-1, pericentriolar material 1

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  WB analysis using 19856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 19856-1-AP

  WB result of PCM1 antibody (19856-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCM1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 19856-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HT-1080 using 19856-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa cells using 19856-1-AP

  IP result of anti-PCM1 (19856-1-AP for IP and Detection) with HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 19856-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 19856-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 19856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 19856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 19856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 19856-1-AP (PCM1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 19856-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCM1 antibody (19856-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 19856-1-AP

  Immunofluorescent analysis of (-20℃Methanol) fixed NIH/3T3 cells using 19856-1-AP (PCM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HT-1080 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human tonsillitis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, NIH/3T3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19856-1-AP targets PCM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13929

  Product name: Recombinant human PCM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-254 aa of BC000453

  Sequence: MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYM

• Full Name: pericentriolar material 1
• Calculated Molecular Weight: 2024 aa, 228 kDa
• Observed Molecular Weight: 260-280 kDa
• GenBank Accession Number: BC000453
• Gene Symbol: PCM1
• Gene ID (NCBI): 5108
• RRID: AB_2878616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15154
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).

Publications

What published studies show

The PCM1 Polyclonal antibody (cat. no. 19856-1-AP) has 36 citations. These appear in journals including Science, Circulation, Nature Communications, EMBO Journal, EMBO Reports, Cell Reports, Journal of Cell Biology, eLife, Journal of Biological Chemistry, Journal of Cell Science, FASEB Journal, and others. Research fields span cell biology and centrosome/centriole biology, ciliogenesis and primary cilium function, cell division, oocyte spindle assembly, cardiomyopathy, atherosclerosis, cancer (breast, renal), inflammation, and developmental disorders. Applications include WB, IF, IHC, and IP.

Automatically generated from the 36 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	WB	Circulation; Jmjd4 Facilitates Pkm2 Degradation in Cardiomyocytes and Is Protective Against Dilated Cardiomyopathy; Authors - Yansong Tang
mouse,human	IF	Sci Bull (Beijing); The mechanisms of MTOCs maturation in human and mouse oocytes.; Authors - Hao Gu
human	WB	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
human	WB,IP	Nat Commun; Lipid droplets sequester palmitic acid to disrupt endothelial ciliation and exacerbate atherosclerosis in male mice; Authors - Yanjie Tan
mouse	IHC	Cardiovasc Diabetol; Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition.; Authors - Charles D Cohen

Reviews

5.0 out of 5

2 verified customer reviews

What customers say

Both reviews rate the antibody 5 stars. One user confirmed it recognized the target protein at the expected molecular weight in Western Blot. Another reported excellent and reliable performance in Immunofluorescence on primary fibroblasts using PFA fixation with methanol permeabilization at a 1:200 dilution. Overall, customers find the antibody specific, consistent, and effective across applications.

Automatically generated from the 2 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Boyan (Verified Customer) (11-25-2024)

recognized a protein at the expected size

• Applications: Western Blot

Julie (Verified Customer) (01-06-2020)

This antibody works great and reliably with PFA fix & methanol permeabilization.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: primary fibroblasts