PCM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PCM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (1)

Cat no : 19856-1-AP

Synonyms

hPCM 1, PCM 1, PCM1, pericentriolar material 1, PTC4

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Validation Data Gallery

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  WB analysis using 19856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 19856-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 19856-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 19856-1-AP

  IP result of anti-PCM1 (19856-1-AP for IP and Detection) with HeLa cells.

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  IHC staining of human tonsillitis using 19856-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 19856-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 19856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 19856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19856-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 19856-1-AP (PCM1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 19856-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCM1 antibody (19856-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red).

• 

  IF Staining of NIH/3T3 using 19856-1-AP

  Immunofluorescent analysis of (-20℃Methanol) fixed NIH/3T3 cells using 19856-1-AP (PCM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 19856-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human PCM1 (19856-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), or 0.2 ug Control Antibody (black). Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, HT-1080 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human tonsillitis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, NIH/3T3 cells, HeLa cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Product Information

The immunogen of 19856-1-AP is PCM1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCM1 fusion protein Ag13929
• Full Name: pericentriolar material 1
• Calculated molecular weight: 2024 aa, 228 kDa
• Observed molecular weight: 260-280 kDa
• GenBank accession number: BC000453
• Gene symbol: PCM1
• Gene ID (NCBI): 5108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).

Publications

Species	Application	Title
human	WB	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
mouse	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran
human	WB	J Cell Biol; A ciliopathy complex builds distal appendages to initiate ciliogenesis.; Authors - Dhivya Kumar ; KO Validated
human,mouse	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall
human	WB	EMBO Rep; Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.; Authors - Melis D Arslanhan
mouse	IHC	Cardiovasc Diabetol; Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition.; Authors - Charles D Cohen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Julie (Verified Customer) (01-06-2020)

This antibody works great and reliably with PFA fix & methanol permeabilization.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: primary fibroblasts