CoraLite® Plus 647-conjugated PCM1 Polyclonal antibody

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  IF Staining of HeLa using CL647-19856

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 647 PCM1 antibody (CL647-19856) at dilution of 1:100.

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  FC experiment of HeLa using CL647-19856

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PCM1 (CL647-19856) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

PCM1 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL647-19856

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

hPCM 1, hPCM-1, PCM 1, PCM-1, pericentriolar material 1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-19856 targets PCM1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13929

  Product name: Recombinant human PCM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-254 aa of BC000453

  Sequence: MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYM

• Full Name: pericentriolar material 1
• Calculated Molecular Weight: 2024 aa, 228 kDa
• Observed Molecular Weight: 260-280 kDa
• GenBank Accession Number: BC000453
• Gene Symbol: PCM1
• Gene ID (NCBI): 5108
• RRID: AB_2934936
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15154
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).