Tested Applications
| Positive WB detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10607-1-AP targets PCOLCE2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0879 Product name: Recombinant human PCOLCE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-276 aa of BC006265 Sequence: MRGANAWAPLCLLLAAATQLSRQQSPERPVFTCGGILTGESGFIGSEGFPGVYPPNSKCTWKITVPEGKVVVLNFRFIDLESDNLCRYDFVDVYNGHANGQRIGRFCGTFRPGALVSSGNKMMVQMISDANTAGNGFMAMFSAAEPNERGDQYCGGLLDRPSGSFKTPNWPDRDYPAGVTCVWHIVAPKNQLIELKFEKFDVERDNYCRYDYVAVFNGGEVNDARRIGKYCGDSPPAPIVSERNELLIQFLSDLSLTADGFIGHYIFRPKKLPTTT Predict reactive species |
| Full Name | procollagen C-endopeptidase enhancer 2 |
| Calculated Molecular Weight | 46 kDa |
| GenBank Accession Number | BC006265 |
| Gene Symbol | PCOLCE2 |
| Gene ID (NCBI) | 26577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKZ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Procollagen C-endopeptidase enhancer 2 (PCOLCE2) is a 415-amino acid protein containing an N-terminal signal sequence, two CUB domains, and an NTR domain. PCOLCE2 binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1. It has been reported that PCOLCE2 is a glycoprotein with an apparent molecular weight of 52 kDa, which is larger than its calculated molecular weight of 45.7 kDa (PMID: 10873381).
Publications
What published studies show
The PCOLCE2 Polyclonal antibody (Cat# 10607-1-AP) has 1 total citation. It appears in PLoS Biology (Impact Factor 6.9), published in January 2022. The cited study investigated colon stroma-mediated inflammation-driven fibroblastic responses controlling matrix remodeling and healing, placing this antibody in the research fields of fibrosis, inflammation, and extracellular matrix biology. The application used was immunofluorescence (IF) in mouse samples.
| Species | Application | Title |
|---|---|---|
PLoS Biol Colon stroma mediates an inflammation-driven fibroblastic response controlling matrix remodeling and healing. |
