PCOLCE2 Polyclonal antibody

PCOLCE2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10607-1-AP

Synonyms

PCOLCE2, PCPE 2, PCPE2

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 10607-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10607-1-AP (PCOLCE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

10607-1-AP targets PCOLCE2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCOLCE2 fusion protein Ag0879
• Full Name: procollagen C-endopeptidase enhancer 2
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: BC006265
• Gene Symbol: PCOLCE2
• Gene ID (NCBI): 26577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Procollagen C-endopeptidase enhancer 2 (PCOLCE2) is a 415-amino acid protein containing an N-terminal signal sequence, two CUB domains, and an NTR domain. PCOLCE2 binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1. It has been reported that PCOLCE2 is a glycoprotein with an apparent molecular weight of 52 kDa, which is larger than its calculated molecular weight of 45.7 kDa (PMID: 10873381).

Publications

Species	Application	Title
mouse	IF	PLoS Biol; Colon stroma mediates an inflammation-driven fibroblastic response controlling matrix remodeling and healing.; Authors - Guadalupe J Jasso