PD-ECGF Recombinant monoclonal antibody

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  WB analysis using 86618-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86618-3-RR (PD-ECGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86618-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86618-3-RR (PD-ECGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86618-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86618-3-RR against Human PD-ECGF were performed. The affinity constant is 1.64 nM.

PD-ECGF Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86618-3-RR

Clone No.251581B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TYMP, EC:2.4.2.4, ECGF1, Gliostatin, Platelet-derived endothelial cell growth factor

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Tested Applications

• Positive WB detected in: A431 cells, THP-1 cells, OVCAR-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86618-3-RR targets PD-ECGF in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3052

  Product name: Recombinant human PD-ECGF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-297 aa of BC018160

  Sequence: MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPAEGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGA

• Full Name: thymidine phosphorylase
• Calculated Molecular Weight: 482 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC018160
• Gene Symbol: Thymidine phosphorylase
• Gene ID (NCBI): 1890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19971
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet derived endothelial cell growth factor (PD-ECGF), also known as thymidine phosphorylase (TP), induces migration and angiogenesis in endothelial and tumor cells, and was upregulated in various malignancies compared to that in normal tissues. Interestingly, PD-ECGF has dual effect on tumor development and chemotherapy. It could stimulate cancer cell migration and proliferation. On the other hand, some chemotherapeutic agents (5-fluorouracil, capecitabine, etc.) were converted to their active forms through TP enzymes.