Alix Polyclonal antibody

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  WB analysis using 12422-1-AP

  human urine exosome samples were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12422-1-AP

  WB result of Alix antibody (12422-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alix transfected HEK-293 cells.

• 

  WB analysis using 12422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12422-1-AP

  IP result of anti-Alix (IP:12422-1-AP, 3ug; Detection:12422-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human liver cancer using 12422-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12422-1-AP (Alix antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12422-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Alix antibody (12422-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Alix Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12422-1-AP

Publications(258)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PDCD6IP, KIAA1375, Hp95, DRIP4, ALG-2-interacting protein X

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Tested Applications

• Positive WB detected in: HEK-293 cells, human urine exosomes tissue, NIH/3T3 cells, HeLa cells, Jurkat cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 243 publications below
• IHC: See 1 publications below
• IF: See 13 publications below
• IP: See 3 publications below

Product Information

12422-1-AP targets Alix in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, monkey, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alix fusion protein Ag3074
• Full Name: programmed cell death 6 interacting protein
• Calculated Molecular Weight: 868 aa, 96 kDa
• Observed Molecular Weight: 96 kDa, 75-80 kDa
• GenBank Accession Number: BC020066
• Gene Symbol: Alix
• Gene ID (NCBI): 10015
• RRID: AB_2162467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WUM4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. The alix antibody recognizes an additional band of 75-80 kDa which has also been observed in cells and exosomes.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human,mouse	WB	Cell Res; Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity; Authors - Ying Gao
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier ; KD Validated
mouse,human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Strong bands appeared between 70 and 100 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse liver tissue

FH

Guorong (Verified Customer) (03-22-2022)

Excellent performance with a specific band of expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: ARPE-19

Alix Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:12422-1-AP)

FH

Susmita (Verified Customer) (02-11-2022)

all antibodies from Proteintech works great for me

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: WB: 1: 2000

FH

Jorge (Verified Customer) (05-15-2019)

Gave a very strong signal using the lowest recommended antibody dilution for Western Blot, diluted it to 1:2000 and worked just as well with a low protein detection substrate. Detected exosomes in fraction 7 of a sucrose gradient as expected.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000 in 5% BSA-TBST
• Cell Tissue Type: Mouse 4T1 cells

Alix Antibody Western Blot, validation (1:1000 in 5% BSA-TBST dilution) in Mouse 4T1 cells (Cat no:12422-1-AP)