|Positive WB detected in||HEK-293 cells, Jurkat cells, mouse liver tissue, rat liver tissue, HeLa cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12422-1-AP targets Alix in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Alix fusion protein Ag3074|
|Full Name||programmed cell death 6 interacting protein|
|Calculated molecular weight||868 aa, 96 kDa|
|Observed molecular weight||96 kDa|
|GenBank accession number||BC020066|
|Gene ID (NCBI)||10015|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.
|Product Specific Protocols|
|WB protocol for Alix antibody 12422-1-AP||Download protocol|
|IHC protocol for Alix antibody 12422-1-AP||Download protocol|
|IF protocol for Alix antibody 12422-1-AP||Download protocol|
|IP protocol for Alix antibody 12422-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Biol Chem
Modulation of calreticulin expression reveals a novel exosome mediated mechanism of Z variant alpha-1 antitrypsin disposal.
Specific Decrease in B-Cell-Derived Extracellular Vesicles Enhances Post-Chemotherapeutic CD8+ T Cell Responses.
The circRNA circPTPRA suppresses epithelial-mesenchymal transitioning and metastasis of NSCLC cells by sponging miR-96-5p.
Cocktail Strategy Based on NK Cell-Derived Exosomes and Their Biomimetic Nanoparticles for Dual Tumor Therapy.
Characterization of urinary extracellular vesicle proteins in muscle-invasive bladder cancer.
J Extracell Vesicles
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jorge (Verified Customer) (05-15-2019)
Gave a very strong signal using the lowest recommended antibody dilution for Western Blot, diluted it to 1:2000 and worked just as well with a low protein detection substrate. Detected exosomes in fraction 7 of a sucrose gradient as expected.