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Alix Antibody 27 Publications

Rabbit Polyclonal| Catalog number: 12422-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 21 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Alix Antibody IHC human liver cancer tissue 12422-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12422-1-AP (Alix antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Alix Antibody IHC human liver cancer tissue 12422-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12422-1-AP (Alix antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Alix Antibody IP Jurkat cells 12422-1-AP

IP Result of anti-PDCD6IP (IP:12422-1-AP, 3ug; Detection:12422-1-AP 1:500) with Jurkat cells lysate 4000ug.

Alix Antibody IF Hela cells 12422-1-AP

Immunofluorescent analysis of Hela cells, using PDCD6IP antibody 12422-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Alix Antibody WB Jurkat cells 12422-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 12422-1-AP( PDCD6IP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, mouse liver tissue, rat liver tissue
Positive IP detected in:
Jurkat cells
Positive IHC detected in:
human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Alix fusion protein Ag3074
Full name:
programmed cell death 6 interacting protein
Calculated molecular weight:
868aa,96 kDa
Observed molecular weight:
96 kDa
GenBank accession number:
BC020066
Gene ID (NCBI):
10015
Gene symbol
PDCD6IP
Synonyms
AIP1, ALG 2 interacting protein 1, Alix, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP

Background

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

Alix Rabbit Polyclonal antibody

Jorge (Verified Customer), 05-15-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000 in 5% BSA-TBST
Alix Antibody Western Blot, validation (1:1000 in 5% BSA-TBST dilution) in Mouse 4T1 cells (Cat no:12422-1-AP)

Alix Antibody Western Blot, validation (1:1000 in 5% BSA-TBST dilution) in Mouse 4T1 cells (Cat no:12422-1-AP)

Cell/Tissue Type
Mouse 4T1 cells
Lot Number
Gave a very strong signal using the lowest recommended antibody dilution for Western Blot, diluted it to 1:2000 and worked just as well with a low protein detection substrate. Detected exosomes in fraction 7 of a sucrose gradient as expected.

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