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  • KD/KO Validated

Alix Polyclonal antibody

Alix Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12422-1-AP

Synonyms

AIP1, ALG 2 interacting protein 1, Alix, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, mouse liver tissue, rat liver tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12422-1-AP targets Alix in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alix fusion protein Ag3074
Full Name programmed cell death 6 interacting protein
Calculated molecular weight 868 aa, 96 kDa
Observed molecular weight 96 kDa
GenBank accession numberBC020066
Gene symbol PDCD6IP
Gene ID (NCBI) 10015
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.

Protocols

Product Specific Protocols
WB protocol for Alix antibody 12422-1-APDownload protocol
IHC protocol for Alix antibody 12422-1-APDownload protocol
IF protocol for Alix antibody 12422-1-APDownload protocol
IP protocol for Alix antibody 12422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Modulation of calreticulin expression reveals a novel exosome mediated mechanism of Z variant alpha-1 antitrypsin disposal.

Authors - Nazli Khodayari
humanWB

Immunity

Specific Decrease in B-Cell-Derived Extracellular Vesicles Enhances Post-Chemotherapeutic CD8+ T Cell Responses.

Authors - Fanghui Zhang
humanWB

EBioMedicine

The circRNA circPTPRA suppresses epithelial-mesenchymal transitioning and metastasis of NSCLC cells by sponging miR-96-5p.

Authors - Siliang Wei
humanWB

Cancers (Basel)

Cocktail Strategy Based on NK Cell-Derived Exosomes and Their Biomimetic Nanoparticles for Dual Tumor Therapy.

Authors - Guosheng Wang
humanWB

Oncotarget

Characterization of urinary extracellular vesicle proteins in muscle-invasive bladder cancer.

Authors - Christopher R Silvers
humanWB,IF

J Extracell Vesicles

Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.

Authors - Wang Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jorge (Verified Customer) (05-15-2019)

Gave a very strong signal using the lowest recommended antibody dilution for Western Blot, diluted it to 1:2000 and worked just as well with a low protein detection substrate. Detected exosomes in fraction 7 of a sucrose gradient as expected.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000 in 5% BSA-TBST
  • Cell Tissue Type: Mouse 4T1 cells
Alix Antibody Western Blot, validation (1:1000 in 5% BSA-TBST dilution) in Mouse 4T1 cells (Cat no:12422-1-AP)