Anti-Human PDGFR beta/CD140b Rabbit Recombinant Antibody

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  FC experiment of MG-63 using 98405-2-RR

  1x10^6 MG-63 cells were surface stained with 0.25 ug Anti-Human PDGFR beta/CD140b Rabbit RecAb (98405-2-RR, Clone: 242711A9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98405-2-RR

  1x10^6 HEK-293T cells or PDGFR beta/CD140b transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human PDGFR beta/CD140b Rabbit RecAb (98405-2-RR, Clone: 242711A9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98405-2-RR

  1x10^6 HEK-293T cells (left) or PDGFR beta/CD140b transfected HEK-293T cells(right) were surface stained with 0.25 ug Anti-Human PDGFRB Rabbit RecAb (98405-2-RR, Clone: 242711A9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

PDGFR beta/CD140b Uni-rAb^® Recombinant Antibody for FC

Cat No. 98405-2-RR

Clone No.242711A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

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Tested Applications

• Positive FC detected in: MG-63 cells, Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98405-2-RR targets PDGFR beta/CD140b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3715

  Product name: recombinant human PDGFRB protein

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 33-531 aa of BC032224

  Sequence: LVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFFGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFK

• Full Name: platelet-derived growth factor receptor, beta polypeptide
• Calculated Molecular Weight: 1106 aa, 124 kDa
• GenBank Accession Number: BC032224
• Gene Symbol: PDGFR beta
• Gene ID (NCBI): 5159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09619
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)