PDI Recombinant antibody

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  WB analysis using 80170-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80170-1-RR (PDI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 80170-1-RR

  WB result of PDI antibody (80170-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells.

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  IHC staining of human prostate cancer using 80170-1-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 80170-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80170-1-RR (PDI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 80170-1-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse lung using 80170-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using PDI antibody (80170-1-RR, Clone: 4K17 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse lung using 80170-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using PDI antibody (80170-1-RR, Clone: 4K17 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 80170-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80170-1-RR against Human PDI were performed. The affinity constant is 13.8 nM.

• Featured Product
• KD/KO Validated

This PDI antibody is a rabbit recombinant antibody that targets PDI in WB, IHC, IF-P, ELISA and is suitable for human, mouse, rat samples.

Cat No. 80170-1-RR

Clone No.4K17

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

P4HB, 4K17, Cellular thyroid hormone-binding protein, CLCRP1, DSI

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human prostate cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

80170-1-RR targets PDI in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDI fusion protein Ag1747
• Full Name: prolyl 4-hydroxylase, beta polypeptide
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC014504
• Gene Symbol: PDI
• Gene ID (NCBI): 5034
• RRID: AB_2918871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07237
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Cancer Prev Res (Phila); Preventive Treatment with PD-1 Antibody Increases Tissue-Resident Memory T Cells Infiltration and Delays Esophageal Carcinogenesis; Authors - Zeru Xiao