PDI Recombinant antibody

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  WB analysis using 80170-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80170-1-RR (PDI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 80170-1-RR

  WB result of PDI antibody (80170-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells.

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  IHC staining of human prostate cancer using 80170-1-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 80170-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80170-1-RR (PDI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 80170-1-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 80170-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80170-1-RR against Human PDI were performed. The affinity constant is 13.8 nM.

• Featured Product
• KD/KO Validated

This PDI antibody is a rabbit recombinant antibody that targets PDI in WB, IHC, ELISA and is suitable for human, mouse, rat samples.

Cat No. 80170-1-RR

Clone No.4K17

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human prostate cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

80170-1-RR targets PDI in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDI fusion protein Ag1747
• Full Name: prolyl 4-hydroxylase, beta polypeptide
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC014504
• Gene Symbol: PDI
• Gene ID (NCBI): 5034
• RRID: AB_2918871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07237
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Cancer Prev Res (Phila); Preventive Treatment with PD-1 Antibody Increases Tissue-Resident Memory T Cells Infiltration and Delays Esophageal Carcinogenesis; Authors - Zeru Xiao