CoraLite® Plus 488-conjugated PDI Recombinant monoclonal antibody

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  IF Staining of mouse lung using CL488-80170

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using CoraLite® Plus 488 PDI antibody (CL488-80170, Clone: 4K17 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

PDI Uni-rAb^® Recombinant Antibody for IF-P

Cat No. CL488-80170

Clone No.4K17

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

P4HB, Cellular thyroid hormone-binding protein, CLCRP1, DSI, EC:5.3.4.1

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Tested Applications

• Positive IF-P detected in: mouse lung tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80170 targets PDI in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1747

  Product name: Recombinant human PDI protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 89-273 aa of BC014504

  Sequence: MTKYKPESEELTAEWITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL

• Full Name: prolyl 4-hydroxylase, beta polypeptide
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC014504
• Gene Symbol: PDI
• Gene ID (NCBI): 5034
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07237
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).