Tested Applications
| Positive WB detected in | HeLa cells, mouse heart tissue, mouse kidney tissue, mouse brain tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 3 publications below |
Product Information
12215-1-AP targets PDK3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2879 Product name: Recombinant human PDK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 129-369 aa of BC015948 Sequence: GVIEYKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERL Predict reactive species |
| Full Name | pyruvate dehydrogenase kinase, isozyme 3 |
| Calculated Molecular Weight | 406 aa, 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC015948 |
| Gene Symbol | PDK3 |
| Gene ID (NCBI) | 5165 |
| RRID | AB_2877836 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15120 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDK3([Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial) is also named as PDHK3 and belongs to the PDK/BCKDK protein kinase family. It inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 and 48 kDa. This protein can exsit as a dimer with the molecular weight of 90 kDa(PMID:11978179).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDK3 antibody 12215-1-AP | Download protocol |
| IP protocol for PDK3 antibody 12215-1-AP | Download protocol |
| WB protocol for PDK3 antibody 12215-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol Overexpression of Pyruvate Dehydrogenase Kinase-3 Predicts Poor Prognosis in Urothelial Carcinoma | ||
Environ Pollut Acrolein exposure affects ovarian function by interfering with glycolysis and mitochondrial energy metabolism in mouse | ||
Mol Cell Biochem Lycorine hydrochloride interferes with energy metabolism to inhibit chemoresistant glioblastoma multiforme cell growth through suppressing PDK3 | ||
Nat Commun NOTCH1 mitochondria localization during heart development promotes mitochondrial metabolism and the endothelial-to-mesenchymal transition in mice | ||
Commun Biol Therapeutic potential of microglial SMEK1 in regulating H3K9 lactylation in cerebral ischemia-reperfusion |
