PDZD7 Polyclonal antibody

• 

  WB analysis using 21084-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21084-1-AP (PDZD7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse eye using 21084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21084-1-AP (PDZD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 21084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21084-1-AP (PDZD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PDZD7 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21084-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PDZK7, PDZ domain-containing protein 7, PDZ domain containing 7

---

Tested Applications

• Positive WB detected in: mouse ear tissue, mouse retina tissue
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21084-1-AP targets PDZD7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15318

  Product name: Recombinant human PDZD7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-319 aa of BC029054

  Sequence: MAQGFAVGFDPLGLGDLSSGSLSSLSSRGHLGSDSGSTATRYLLRKQQRLLNGPPRGIRASSPMGRVILINSPIEANSDESDIIHSVRVEKSPAGRLGFSVRGGSEHGLGIFVSKVEEGSSAERAGLCVGDKITEVNGLSLESTTMGSAVKVLTSSSRLHMMVRRMGRVPGIKFSKEKTTWVDVVNRRLVVEKCGSTPSDTSSEDGVRRIVHLYTTSDDFCLGFNIRGGKEFGLGIYVSKVDHGGLAEENGIKVGDQVLAANGVRFDDISHSQAVEVLKGQTHIMLTIKETGRYPAYKEMVSEYCWLDRLSNGVLQQLS

• Full Name: PDZ domain containing 7
• Calculated Molecular Weight: 517 aa, 56 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC029054
• Gene Symbol: PDZD7
• Gene ID (NCBI): 79955
• RRID: AB_3085636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H5P4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDZD7, also known as PDZK7, is essential in organizing the USH2 complex at stereocilia ankle links and blocks inhibition of adenylate cyclase activity mediated by ADGRV1. PDZD7 encodes a PDZ domain-containing scaffold protein that is highly expressed in hair cells of the inner ear. PDZD7 is also expressed in the cilia of photoreceptors (PMID: 33530996). Dysfunction or mutations of PDZD7 result in Usher syndrome type 2 (USH2) and Autosomal recessive non-syndromic hearing loss(ARNSHL) (PMID: 29048736, 20440071).