PEG10 Polyclonal antibody

PEG10 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 14412-1-AP

Synonyms

Edr, HB 1, KIAA1051, Mar2, Mart2, MEF3 like protein 1, MEF3L, MEF3L1, paternally expressed 10, PEG10, RGAG3, Ty3/Gypsy like protein

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Validation Data Gallery

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  WB analysis using 14412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14412-1-AP (PEG10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14412-1-AP (PEG10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 14412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14412-1-AP (PEG10 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse testis using 14412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14412-1-AP (PEG10 Antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of mouse testis using 14412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14412-1-AP (PEG10 Antibody) at dilution of 1:25 (under 40x lens).

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  IHC staining of human placenta using 14412-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14412-1-AP (PEG10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14412-1-AP (PEG10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 14412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14412-1-AP (PEG10 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 14412-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PEG10 antibody (14412-1-AP) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, PC-13 cells, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, mouse testis tissue, mouse brain tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

14412-1-AP targets PEG10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEG10 fusion protein Ag5754
• Full Name: paternally expressed 10
• Calculated molecular weight: 80 kDa
• Observed molecular weight: 50 kDa, 100 kDa
• GenBank accession number: BC050659
• Gene symbol: PEG10
• Gene ID (NCBI): 23089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,IF	Cell; Cellular architecture of human brain metastases.; Authors - Hugo Gonzalez
human	WB	Acta Pharmacol Sin; Transcriptional regulator CTR9 promotes hepatocellular carcinoma progression and metastasis via increasing PEG10 transcriptional activity.; Authors - Bin Zhang
human	IF	Elife; UBQLN2 restrains the domesticated retrotransposon PEG10 to maintain neuronal health in ALS; Authors - Holly H Black
human	WB	Ann Med; A 12-gene panel in estimating hormone-treatment responses of castration-resistant prostate cancer patients generated using a combined analysis of bulk and single-cell sequencing data; Authors - Juanlan Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jordan (Verified Customer) (09-03-2019)

The antibody works well in our condition. MCF-7 cell lines. 1/1000 in TBS-T 5%BSA.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000