PELO Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85743-1-RR (same clone as 85743-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85743-1-RR (PELO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85743-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85743-1-RR (same clone as 85743-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PELO antibody (85743-1-RR, Clone: 243137F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85743-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02111-1

  Cytometric bead array standard curve of MP02111-1, PELO Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85743-1-PBS. Detection antibody: 85743-2-PBS. Standard: Ag0873. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 85743-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85743-1-RR against Human PELO were performed. The affinity constant is below 1 pM.

PELO Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 85743-1-PBS

Clone No.243137F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

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Product Information

85743-1-PBS targets PELO as part of a matched antibody pair:

MP02111-1: 85743-1-PBS capture and 85743-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PELO fusion protein Ag0873
• Full Name: pelota homolog (Drosophila)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC005889
• Gene Symbol: PELO
• Gene ID (NCBI): 53918
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BRX2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].