PEPD Monoclonal antibody

• Home
• Products
• Primary Antibodies
• PEPD Monoclonal antibody (67202-1-Ig)

PEPD Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67202-1-Ig Copied!

Clone No. 1H2A1

Publications (1)

Download datasheet

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA
For IF, select conjugated version.

Synonyms

1H2A1, EC:3.4.13.9, PRD, PROLIDASE, Xaa-Pro dipeptidase

• 

  WB analysis using 67202-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NCCIT using 67202-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 67202-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 67202-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 67202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 67202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for PEPD in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, human placenta tissue, NCCIT cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67202-1-Ig targets PEPD in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28713

  Product name: Recombinant human PEPD protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 192-439 aa of BC015027

  Sequence: LEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREV

• Full Name: peptidase D
• Calculated Molecular Weight: 493 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC015027
• Gene Symbol: PEPD
• Gene ID (NCBI): 5184
• RRID: AB_2882495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P12955
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)

Publications

What published studies show

The PEPD Monoclonal antibody (1H2A1), catalog number 67202-1-Ig, has a total of 1 citation. This citation appears in Frontiers in Bioengineering and Biotechnology (Impact Factor 5.8), published in December 2021. The study focused on small-molecule induction promoting corneal endothelial cell differentiation from human induced pluripotent stem cells (iPS cells), utilizing the antibody for Western blot analysis in human samples. The research field encompasses stem cell biology, bioengineering, and ocular tissue regeneration.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Bioeng Biotechnol; Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells.; Authors - Jie Chen