PEPD Monoclonal antibody, PBS Only

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  WB analysis using 67202-1-Ig (same clone as 67202-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 67202-1-Ig (same clone as 67202-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67202-1-Ig (same clone as 67202-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67202-1-Ig (same clone as 67202-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67202-1-Ig (same clone as 67202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67202-1-Ig (same clone as 67202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

PEPD Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67202-1-PBS

Clone No.1H2A1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

Imidodipeptidase, PEPD, peptidase D, PRD, PROLIDASE, Proline dipeptidase, X Pro dipeptidase, Xaa Pro dipeptidase

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Product Information

67202-1-PBS targets PEPD in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28713

  Product name: Recombinant human PEPD protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 192-439 aa of BC015027

  Sequence: LEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREV

• Full Name: peptidase D
• Calculated Molecular Weight: 493 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC015027
• Gene Symbol: PEPD
• Gene ID (NCBI): 5184
• RRID: AB_2882495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P12955
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)