|Positive WB detected in||Jurkat cells, K-562 cells, mouse heart tissue|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14713-1-AP targets PEX19 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||Arabidopsis, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PEX19 fusion protein Ag6434|
|Full Name||peroxisomal biogenesis factor 19|
|Calculated molecular weight||33 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||BC000496|
|Gene ID (NCBI)||5824|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).
The Early-Acting Peroxin PEX19 Is Redundantly Encoded, Farnesylated, and Essential for Viability in Arabidopsis thaliana.
Cell Death Differ
Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.
Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (09-08-2021)
Very good antibody - no non-specific bands. Membrane blocked with BSA in TBS.