PEX19 Polyclonal antibody

PEX19 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14713-1-AP

Synonyms

33 kDa housekeeping protein, D1S2223E, HK33, Peroxin 19, PEX19, PMP1, PMPI, PXF, PXMP1



Tested Applications

Positive WB detected inJurkat cells, K-562 cells, mouse heart tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

14713-1-AP targets PEX19 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited ReactivityArabidopsis, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX19 fusion protein Ag6434
Full Name peroxisomal biogenesis factor 19
Calculated molecular weight 33 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC000496
Gene symbol PEX19
Gene ID (NCBI) 5824
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).

Protocols

Product Specific Protocols
WB protocol for PEX19 antibody 14713-1-APDownload protocol
IHC protocol for PEX19 antibody 14713-1-APDownload protocol
IP protocol for PEX19 antibody 14713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ArabidopsisWB

PLoS One

The Early-Acting Peroxin PEX19 Is Redundantly Encoded, Farnesylated, and Essential for Viability in Arabidopsis thaliana.

Authors - Margaret M McDonnell
humanWB

Cell Death Differ

Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.

Authors - Weiwei Cui
mouseWB

Nat Commun

Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling.

Authors - Celia de la Calle Arregui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (09-08-2021)

Very good antibody - no non-specific bands. Membrane blocked with BSA in TBS.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293
PEX19 Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:14713-1-AP)