PFKFB2 Polyclonal antibody

PFKFB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17838-1-AP


6PF 2 K/Fru 2,6 P2ase 2, PFK 2/FBPase 2, PFK/FBPase 2, PFKFB2

Tested Applications

Positive WB detected inhuman heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

17838-1-AP targets PFKFB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFKFB2 fusion protein Ag12181
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Calculated Molecular Weight 505 aa, 58 kDa
Observed Molecular Weight 58 kDa, 110 kDa
GenBank Accession NumberBC075076
Gene Symbol PFKFB2
Gene ID (NCBI) 5208
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PFKFB2(6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase 2) is also named as PFK-2/FBPase-2, which is a bifunctional enzyme responsible for the synthesis and breakdown of Fru-2,6-P2 in the regulation of glycolysis(PMID:10095107). In the C-terminal section, it belongs to the phosphoglycerate mutase family.The enzyme is a dimer of identical subunits, each composed of 505 amino acids and containing three discrete domains(PMID: 2552438). It has 2 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for PFKFB2 antibody 17838-1-APDownload protocol
IHC protocol for PFKFB2 antibody 17838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control

Authors - Meiling Wu

Cell Death Dis

SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway.

Authors - Shu-Jie Zhao

Free Radic Biol Med

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Authors - Kelei Dong

J Biol Chem

Type 2 Diabetes Dysregulates Glucose Metabolism in Cardiac Progenitor Cells.

Authors - Joshua K Salabei

Int J Biochem Cell Biol

A transcriptional target of androgen receptor, miR-421 regulates proliferation and metabolism of prostate cancer cells.

Authors - Delong Meng

J Transl Med

Small molecule inhibition of 6-phosphofructo-2-kinase suppresses t cell activation.

Authors - Telang Sucheta S