PGBD1 Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 25539-1-AP (same clone as 25539-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25539-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 25539-1-AP (same clone as 25539-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25539-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 25539-1-AP (same clone as 25539-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25539-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 25539-1-AP (same clone as 25539-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25539-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 25539-1-AP (same clone as 25539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25539-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 25539-1-AP (same clone as 25539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25539-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 25539-1-AP (same clone as 25539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25539-1-AP (PGBD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25539-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 25539-1-AP (same clone as 25539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25539-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 25539-1-AP (same clone as 25539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25539-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 25539-1-AP (same clone as 25539-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGBD1 antibody (25539-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25539-1-PBS in a different storage buffer formulation.

PGBD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 25539-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SCAND4, PiggyBac transposable element-derived protein 1, PGBD 1, hucep-4, HUCEP4

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Product Information

25539-1-PBS targets PGBD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGBD1 fusion protein Ag19837
• Full Name: piggyBac transposable element derived 1
• Calculated Molecular Weight: 809 aa, 93 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC128585
• Gene Symbol: PGBD1
• Gene ID (NCBI): 84547
• RRID: AB_2880123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96JS3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.