PGBD1 Polyclonal antibody

PGBD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25539-1-AP

Synonyms

SCAND4, PiggyBac transposable element-derived protein 1, PGBD 1, hucep-4, HUCEP4

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Validation Data Gallery

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  WB analysis of HeLa using 25539-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25539-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 25539-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25539-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 25539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 25539-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 25539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25539-1-AP (PGBD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 25539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25539-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 25539-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGBD1 antibody (25539-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, mouse liver tissue
• Positive IHC detected in: mouse testis tissue, human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25539-1-AP targets PGBD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGBD1 fusion protein Ag19837
• Full Name: piggyBac transposable element derived 1
• Calculated Molecular Weight: 809 aa, 93 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC128585
• Gene Symbol: PGBD1
• Gene ID (NCBI): 84547
• RRID: AB_2880123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.