Tested Applications
| Positive WB detected in | A375 cells, A549 cells, HL-60 cells, K-562 cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human pancreas cancer tissue, human colon tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12867-1-AP targets PHC2 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3507 Product name: Recombinant human PHC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-323 aa of BC028396 Sequence: MTSGNGNSASSIAGTAPQNGENKPPQAIVKPQILTHVIEGFVIQEGAEPFPVGRSSLLVGNLKKKYAQGFLPEKLPQQDHTTTTDSEMEEPYLQESKEEGAPLKLKCELCGRVDFAYKFKRSKRFCSMACAKRYNVGCTKRVGLFHSDRSKLQKAGAATHNRRRASKASLPPLTKDTKKQPTGTVPLSVTAALQLTHSQEDSSRCSDNSSYEEPLSPISASSSTSRRRQGQRDLELPDMHMRDLVGMGHHFLPSEPTKWNVEDVYEFIRSLPGCQEIEEEFRAQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARISMLKDS Predict reactive species |
| Full Name | polyhomeotic homolog 2 (Drosophila) |
| Calculated Molecular Weight | 858 aa, 91 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC028396 |
| Gene Symbol | PHC2 |
| Gene ID (NCBI) | 1912 |
| RRID | AB_2877890 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IXK0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PHC2 antibody 12867-1-AP | Download protocol |
| IHC protocol for PHC2 antibody 12867-1-AP | Download protocol |
| IP protocol for PHC2 antibody 12867-1-AP | Download protocol |
| WB protocol for PHC2 antibody 12867-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody anti-PHC2 (Cat# 12867-1-AP) has 4 total citations. These appear in Nature Genetics, Molecular Cell (×2), and Cell Signaling. The research fields span chromatin biology, PRC1 condensate dynamics, developmental gene regulation, and hepatocellular carcinoma. Applications cited include WB, IF, ChIP, and IHC across human and mouse species.
| Species | Application | Title |
|---|---|---|
Nat Genet H3K27me3 spreading organizes canonical PRC1 chromatin architecture to regulate developmental programs. | ||
Mol Cell Mutual antagonism between PRC1 condensates and SWI/SNF in chromatin regulation.
| ||
Mol Cell Modularity of PRC1 composition and chromatin interaction define condensate properties | ||
Cell Signal PHC2 promotes hepatocellular carcinoma progression and serves as a robust prognostic biomarker: A pancancer multiomics and clinical validation study. |
