PHC2 Polyclonal antibody

PHC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12867-1-AP

Synonyms

Polyhomeotic-like protein 2, Polyhomeotic like protein 2, PH2, HPH2, EDR2

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Validation Data Gallery

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  WB analysis of A375 using 12867-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12867-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 12867-1-AP

  IP result of anti-PHC2 (IP:12867-1-AP, 4ug; Detection:12867-1-AP 1:500) with HL-60 cells lysate 4000 ug.

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  IHC staining of human pancreas cancer using 12867-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12867-1-AP (PHC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12867-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12867-1-AP (PHC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12867-1-AP (PHC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12867-1-AP (PHC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A375 using 12867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12867-1-AP (PHC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, A549 cells
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human pancreas cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

12867-1-AP targets PHC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHC2 fusion protein Ag3507
• Full Name: polyhomeotic homolog 2 (Drosophila)
• Calculated Molecular Weight: 858 aa, 91 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC028396
• Gene Symbol: PHC2
• Gene ID (NCBI): 1912
• RRID: AB_2877890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Mol Cell; Modularity of PRC1 composition and chromatin interaction define condensate properties; Authors - Stefan Niekamp