PHF10 Monoclonal antibody

PHF10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

4D4C6

Cat no : 66341-1-Ig

Synonyms

BAF45a, BRG1 associated factor 45a, PHD finger protein 10, PHF10, XAP135

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Validation Data Gallery

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  WB analysis of fetal human brain using 66341-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66341-1-Ig (PHF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66341-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66341-1-Ig (PHF10 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66341-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66341-1-Ig (PHF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66341-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66341-1-Ig (PHF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 66341-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66341-1-Ig (PHF10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66341-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66341-1-Ig (PHF10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, HEK-293 cells, Neuro-2a cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

66341-1-Ig targets PHF10 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PHF10 fusion protein Ag19096
• Full Name: PHD finger protein 10
• Calculated Molecular Weight: 408 aa, 46 kDa
• Observed Molecular Weight: 56 kDa, 35 kDa
• GenBank Accession Number: BC020954
• Gene Symbol: PHF10
• Gene ID (NCBI): 55274
• RRID: AB_2881721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHF10, also named as BRG1-associated factor 45a, is a 498 amino acid protein, which locate in the nucleus and Belongs to the SAYP family. PHF10 Involve in transcription activity regulation by chromatin remodeling. It Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. PHF10 exists as several isoform and the calculated molecular weight of each isoform is 42 kDa, 37 kDa, 51 kDa, and 56 kDa.

Publications

Species	Application	Title
mouse	WB	Glia; DHHC5 facilitates oligodendrocyte development by palmitoylating and activating STAT3.; Authors - Yanchen Ma
human	CoIP	bioRxiv; BRD9-SMAD2/3 orchestrates stemness and tumorigenesis in pancreatic ductal adenocarcinoma; Authors - Yuliang Feng
human,mouse	IF	Gastroenterology; BRD9-SMAD2/3 orchestrates stemness and tumorigenesis in pancreatic ductal adenocarcinoma; Authors - Yuliang Feng