PI3 Kinase p110 Alpha Polyclonal antibody

• 

  WB analysis using 27921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3 Kinase p110 Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PI3 Kinase p110 Alpha Polyclonal Antibody for WB, ELISA

Cat No. 27921-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, CoIP, ELISA

Synonyms

PI3K p110(alpha), PIK3CA, EC:2.7.1.137, EC:2.7.11.1, p110 alpha

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 24 publications below
• CoIP: See 1 publications below

Product Information

27921-1-AP targets PI3 Kinase p110 Alpha in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27539

  Product name: Recombinant human PIK3CA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 345-550 aa of BC113601

  Sequence: NVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDF

• Full Name: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Calculated Molecular Weight: 1068 aa, 124 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC113601
• Gene Symbol: PIK3CA
• Gene ID (NCBI): 5290
• RRID: AB_2918135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42336
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	J Ethnopharmacol; Xiao-Luo-Wan treats propylthiouracil-induced goiter with hypothyroidism in rats through the PI3K-AKT/RAS pathways based on UPLC/MS and network pharmacology.; Authors - Shi Dong
rat	WB	Fitoterapia; Integrating LC-MS and molecular docking technology to investigate the effect of Spatholobi caulis flavonoids in alleviating primary dysmenorrhea via regulating the RhoA/ROCK pathway; Authors - Xuan Zhang
mouse,human	WB	Heliyon; Anticancer potential of grifolin in lung cancer treatment through PI3K/AKT pathway inhibition; Authors - Li Wang
rat	WB,CoIP	J Adv Res; PTPN22 as a therapeutic target in intervertebral disc degeneration: Modulating mitophagy and pyroptosis through the PI3K/AKT/mTOR axis; Authors - Haibo Liang
human	WB	J Biochem Mol Toxicol; SPAG4 enhances mitochondrial respiration and aerobic glycolysis in colorectal cancer cells by activating the PI3K/Akt signaling pathway; Authors - Jiehao Zhou
mouse	WB	J Ethnopharmacol; Dendrobium huoshanense in the treatment of ulcerative colitis: Network pharmacology and experimental validation; Authors - Liang Yao