Tested Applications
| Positive WB detected in | HeLa cells, Hepa1-6 cells, HepG2 cells, hTERT-RPE1 cells |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28511-1-AP targets Piezo1 (extracellular domain) in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29858 Product name: Recombinant human FAM38A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 108-294 aa of BC008073 Sequence: YEELSRQFDPQPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDK Predict reactive species |
| Full Name | family with sequence similarity 38, member A |
| Calculated Molecular Weight | 286 kDa |
| Observed Molecular Weight | 280-300 kDa |
| GenBank Accession Number | BC008073 |
| Gene Symbol | Piezo1/FAM38A |
| Gene ID (NCBI) | 9780 |
| RRID | AB_2881161 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92508 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells which influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as PIEZO1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Fam38A also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in gene encoding PIEZO1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Piezo1 (extracellular domain) antibody 28511-1-AP | Download protocol |
| WB protocol for Piezo1 (extracellular domain) antibody 28511-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Piezo1 (extracellular domain) Rabbit Polyclonal Antibody (Cat# 28511-1-AP) has 39 citations published in journals including Cell, Nature Communications, Neuron, Nature Materials, Science Advances, ACS Nano, Bioactive Materials, JCI Insight, and FASEB Journal. Research fields span mechanotransduction, fibrosis (renal, endometrial, bladder), oncology (ovarian, hepatocellular, glioblastoma), neurodegeneration (Alzheimer's), cardiovascular disease, bone biology, diabetes, and inflammatory disorders.
| Species | Application | Title |
|---|---|---|
Bioact Mater Dynamically responsive hydrogel with mechanical stimulation enhances diabetic wound healing via activation of Piezo1-mediated efferocytosis. | ||
Nat Commun Environmental stiffness regulates neuronal maturation via Piezo1-mediated transthyretin activity.
| ||
ACS Nano Static Topographical Cue Combined with Dynamic Fluid Stimulation Enhances the Macrophage Extracellular Vesicle Yield and Therapeutic Potential for Bone Defects |
