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Piezo1 (extracellular domain) Polyclonal antibody
Piezo1 (extracellular domain) Polyclonal Antibody for IHC, WB, ELISA
Cat no : 28511-1-AP
|Positive WB detected in||HeLa cells, HepG2 cells, hTERT-RPE1 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
28511-1-AP targets Piezo1 (extracellular domain) in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Piezo1 (extracellular domain) fusion protein Ag29858|
|Full Name||family with sequence similarity 38, member A|
|Calculated molecular weight||286 kDa|
|Observed molecular weight||280-300 kDa|
|GenBank accession number||BC008073|
|Gene ID (NCBI)||9780|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells which influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as PIEZO1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Fam38A also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in gene encoding PIEZO1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1.
Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes
Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells.
Mechanosensitive Piezo1 channels mediate renal fibrosis.
The function of Piezo1 in hepatoblastoma metastasis and its potential transduction mechanism
Microglial Piezo1 senses Aβ fibril stiffness to restrict Alzheimer's disease